After the defeat of their old arch nemesis, The Shredder, the Turtles have grown apart as a family. Struggling to keep them together, their rat sensei, Splinter, becomes worried when strange things begin to brew in New York City.

Chris Evans
as Casey Jones (voice)

Sarah Michelle Gellar
as April O'Neil (voice)

Patrick Stewart
as Winters (voice)

Mako
as Splinter (voice)

Zhang Ziyi
as Karai (voice)

James Arnold Taylor
as Leonardo (voice)

Mitchell Whitfield
as Donatello / Additional Voices (voice)

Nolan North
as Raphael / Nightwatcher (voice)

Mikey Kelley
as Michelangelo (voice)

Laurence Fishburne
as Narrator (voice)