A psychiatrist tells two stories: one of a trans woman, the other of a pseudohermaphrodite.

Bela Lugosi
as Scientist

Edward D. Wood Jr.
as Glen / Glenda (as Daniel Davis)

Lyle Talbot
as Inspector Warren

Timothy Farrell
as Dr. Alton / Narrator

Dolores Fuller
as Barbara

'Tommy' Haynes
as Alan / Anne

Charlie Crafts
as Johnny (as Charles Crafts)

Conrad Brooks
as Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Henry Bederski
as Man with Hat and Receding Hairline (uncredited)

Carol Daugherty
as Woman in Nightmare (uncredited)